Plasmid name :
pLKO.1-mGsk3b shRNA (3+1) Gene/insert name :
Gsk3b shRNA Alt name :
GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik Insert size :
~60bp Species :
Mus musculus (house mouse) Gene ID :
56637 Accessions :
N/A Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
pLKO.1-mGsk3b shRNA (3+1) Gene/insert name :
Gsk3b shRNA Alt name :
GSK3; GSK-3; C86142; GSK-3beta; 7330414F15Rik; 8430431H08Rik Insert size :
~60bp Species :
Mus musculus (house mouse) Gene ID :
56637 Accessions :
N/A Vector Backbone :
pLKO.1-Puro Tag :
None Backbone manufacturer :
Addgene Vector type :
Lentiviral interference Backbone size w/o insert :
7091bp Cloning site 5' :
Age I Site destroyed during cloning :
YES Cloning site 3' :
EcoR I Site destroyed during cloning :
NO Bacterial resistance(s) :
Ampicillin Growth strain(s) :
Stbl3 Growth temperature :
37°C High or low copy :
High Copy Plasmid description :
This product consists of three shRNA constructs to ensure effective silencing of the target gene, as well as a scramble shRNA. Selectable markers :
Puromycin
Blocking peptide available as PPL50075-3P