Product Name :
DAPI Staining Kit Applications :
This product is 1mg/ml Chromogen. Solute it with suitable density for applies. Recommend density for tissue staining is 2 ug/ml（on the 0.5ug/ml, flourescence become light saturation）. For cell culture the density is 0.1ug/ml. Background :
DAPI (4',6-diamidino-2-phenylindole) is cell permeable fluorescent minor groove-binding probe for DNA; it binds to the double-stranded DNA (especially to AT rich DNA), and forming a stable fluoresces complex. DAPI throughout the live and dead cell membrane that can be utilized for DAN detect for chromosome DAN, Yeast DNA, chloroplast DNA, Virus DNA and so on. DAPI-DNA complex shows light blue flourescence color with excitation light 364 nm and emission light 454 nm. Alternative Name :
1. The DAPI is faded with light, all experiment process need keep away from light; 2. For research only, not intended to clinical diagnosis. Classification :
IHC Related Reagents Format :
Kit Contents :
A:DAPI Chromogen (1mg/ml) 0.1ml B:Dilution Buffer 100ml Storage & Shelf Life :
Store at 2-8°C; the chromogen store at -20°C. Each component is stable for up to 12 Months. Procedure :
1. For double or triple fluorescence staining in immunofluorescence tests, the DAPI staining is the last step after all fluorescence antibodies incubation; 2. For tissue cell staining, apply 20 ul Chromogen to 10 ml Dilution Buffer in tube and mix (the end density is 2ug/ml); incubate the tissue with DAPI buffer about 15-30min, at 30℃; and then wash it with PBS/TBS for 3 times; 3. For culture cell, the DAPI density is 0.5ug/ml (apply 5 ul Chromogen to 10 ml Dilution Buffer) may be suitable; 4. Cover the microscope cover glass for observation (NOTE: It’s better for observation and operation when the section is dried in no dark box before adding anti-fading buffer). Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :

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Blocking peptide available as BD5010P